Develop and validate better methods for veterinary drug residues. Perform bridging studies between the new and existing regulatory residue methods. This project will provide more rapid and reliable methods to detect unsafe veterinary drug residues in animal-derived food products. The improved methods will enhance regulatory agencies' ability to detect unsafe animal-derived food products. (1) ON TRACK ON TRACK main 1. Conduct method validation for: sub a. Ceftiofur metabolite (marker residue) in ruminant tissues (with an emphasis on kidney, the target issue) 5/31/2011 Completed 5/31/2011 sub b. Chlortetracycline in ruminant tissues (with an emphasis on kidney, the target issue) 6/30/2011 Completed 5/31/2011 sub c. Tetracycline in ruminant tissues (with an emphasis on kidney, the target issue) 12/31/2011 Completed main 2. Develop research protocol for ceftiofur: sub a. Develop draft research protocol 1/31/2011 Completed 1/31/2011 sub b. Complete review and approval of research protocol 3/15/2011 Completed 3/15/2011 main 3. Complete the pilot studies for: sub a. Chlortetracycline 5/3/2011 Completed 4/29/2011 sub b. Ceftiofur 7/31/2011 Completed 7/31/2011 sub c. Tetracycline 10/31/2011 <i>(12/30/2011)</i> Completed 12/30/2011 main 4. Complete the main phases of the bridging studies for: sub a. Chlortetracycline 12/31/2011 Completed 12/31/2011 sub b. Ceftiofur 12/31/2011 <i>(3/30/2012)</i> <i>(5/31/2012)</i> Completed 6/15/2012 sub c. Tetracycline 4/30/2012 <i>(7/31/2012)</i> Completed 6/15/2012 main 5. Complete assessment of research findings and reports 6/30/2012 <i>(12/30/2012)</i> <i>(1/31/2013)</i> <i>(5/30/2013)</i> On Track main 6. Determined the candidate drugs for future bridging studies 6/30/2012 <i>(12/30/2012)</i> Completed 12/3/2012 main 7. Submit methods paper manuscript for publication 9/30/2012 Completed 5/30/2012 (1) The dates in italics under the milestone due dates are modified due dates which had to be updated due to real-time delays. The milestone status reflects the modified dates. Ceftiofur antimicrobial agent Chlortetracycline antimicrobial agent Tetracycline antimicrobial agent protocol guidelines In vivo in the living organism http://www.fda.gov/AboutFDA/Transparency/track/ucm203425.htm FDA-TRACK CVM Dashboard http://www.fda.gov/AnimalVeterinary/ScienceResearch/ResearchAreas/ucm109129.htm CVM OR Research Highlights ਍††਍਍਍਍਍਍਍