Identify biologically active substances that have efficacy to treat or prevent the development of severe infections. Antibiotic resistance developed from agribusiness use of antibiotics in the food chain is a continuing problem. NCTR research includes the evaluation of the incidence and mechanisms of drug resistance. As FDA sets antimicrobial drug-residue limits in animal products, this knowledge can be used to assist the agency to establish better strategies for antibiotic use. This information can also be used to help determine the contribution of different antimicrobials used in the food-production environment to the spread of multi-drug resistance and increased virulence of foodborne pathogens, and can be used to aid risk assessments. These studies will help address the need of alternative approaches to deal with multidrug resistant pathogens. main 1. Develop a comprehensive protein database for pathogenic Staphylococcus aureus (S. aureus) isolates 4/30/2010 <i>(7/31/2010)</i> Completed 7/31/2010 main 2. Identify proteins from S. aureus that may be involved in virulence and pathogenicity 5/31/2010 <i>(8/31/2010)</i> Completed 8/31/2010 main 3. Examine the role of hyaluronidases as a potential virulence factors in S. aureus 6/30/2010 <i>(9/30/2010)</i> <i>(9/30/2011)</i> Completed 9/30/2011 main 4. Complete the assessment of research findings 6/30/2011 <i>(10/31/2011)</i> Completed 10/15/2011 main 5. Develop draft technical report/manuscript/publication 7/31/2011 <i>(12/31/2011)</i> Completed 7/31/2011 main 6. Complete the peer review (NCTR and other researchers) of draft technical report, manuscript, and/or publication 3/31/2012 Completed 7/31/2011 main 7. Obtain NCTR Center Director approval on the draft technical report, manuscript, and/or publication via the document tracking system 6/30/2012 Completed 7/31/2011 main 8. Submit final technical report, manuscript and/or publication to a scientific journal 6/30/2012 Completed 6/30/2012 http://www.fda.gov/AboutFDA/Transparency/track/ucm203296.htm FDA-TRACK NCTR Dashboard http://www.fda.gov/AboutFDA/Transparency/track/ucm252974.htm FDA-TRACK Research Glossary S. aureus most common cause of staph infections pathogenicity ability of a pathogen to cause disease hyaluronidases family of enzymes that degrade hyaluronic acid increasing tissue permeability and may help bacterial cells spread from their primary site of infection ਍††਍਍਍਍਍਍਍