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U.S. Department of Health and Human Services

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Title:  Comparative efficacy of MS-222 and benzocaine as anaesthetics under simulated transport conditions of a tropical ornamental fish Puntius filamentosus (Valenciennes).
 
Authors:  Pramod PK, Ramachandran A, Sejeevan TP, Thampy S, Pai SS
 
Drug:  Benzocaine
 
Fish species:  Barb, blackspot
 
Date:  2010
 
Sample:  blood, plasma
 
Ave Weight (gm):  12
 
Ave Water temp (°C):  22
 
Water:  Freshwater
 
Dosage:  20 mg/L 48h
 
Route:  BATH
 
Comments:  Fish were kept for 48h in water at 22 C and after sampling, remaining fish were transfered to tanks with water at 28 C.Fish euthanized with lethal dose concentration of 200 mg/L of MS-222 before sampling. The lowest increase in plasma cortisol levels was in MS-222 treated group followed by benzocain treatment within the first 6h of packing. Increase significantly lower (P<0.05) than nonsedated fish however no significant difference between MS-222 and benzocaine. Increase in blood glucose was significantly lower than nonsedated fish but not significantly different than the MS-222 treatment. Post transport mortality after 7d was 20% compared to 43% in nonsedated fish.
 
Citation:  Aquaculture Research 41:309-314
 
Link:  http://dx.doi.org/10.1111/j.1365-2109.2009.02333.x  Opens a new window
 
Genus Species:  Puntius filamentosus
 
Drug Class:  Anesthetic-Benzocaine
 
Wt gm-detail:  12+/- 1g
 
Water temp (°C)-detail:  48 h:22+/-1C, 7d:28+/-1C
 
Dosage-detail:  20 mg/L
 
Method:  ELISA
 
Authors-red:  Pramod PK, Ramachandran A, Sejeevan TP, Thampy S, Pai SS
 
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