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U.S. Department of Health and Human Services

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TITLE:  Antimicrobial susceptibility of Staphylococcus aureus from retail ground meats
AUTHORS:  Kelman A;Soong YA;Dupuy N;Shafer D;Richbourg W;Johnson K;Brown T;Kestler E;Li Y;Zheng J;McDermott P;Meng J;
YEAR:  2011
JOURNAL FULL:  Journal of food protection
ISSUE:  10
END PAGE:  1629
KEYWORDS:  Ampicillin;analysis;Animals;antimicrobial;ANTIMICROBIAL RESISTANCE;Antimicrobials;Cattle;Chloramphenicol;Clindamycin;Colony Count,Microbial;Consumer Product Safety;Dose-Response Relationship,Drug;drug effects;Drug Resistance,Bacterial;Drug Resistance,Multiple,Bacterial;Erythromycin;Food;Food Contamination;Food Supply;GENE;GENTAMICIN;GROUND-BEEF;Humans;isolation & purification;Maryland;Meat;Meat Products;Methicillin-Resistant Staphylococcus aureus;Microbial Sensitivity Tests;microbiology;Research;retail meat;SINGLE;Staphylococcus;Staphylococcus aureus;STAPHYLOCOCCUS-AUREUS;SUSCEPTIBILITY;Swine;Tetracycline;Turkeys;United States;UNITED-STATES;
ABSTRACT:  The aim of this study was to characterize antimicrobial resistance in Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA), recovered from raw retail meat products purchased in the Washington, D.C., area. From March to August 2008, 694 samples of ground beef (n = 198), ground pork (n = 300), and ground turkey (n = 196) were collected by random sampling from stores of three grocery chains. In total, 200 S. aureus isolates (29%) were recovered by direct plating. When tested for susceptibility to 22 antimicrobials, 69% of the S. aureus isolates were resistant to tetracycline, 26% to penicillin, 17% to ampicillin, 13% to methicillin, 8% to erythromycin, 4.5% to clindamycin, 1.5% to gentamicin, and 0.5% to chloramphenicol, oxacillin, cefoxitin, or quinupristin-dalfopristin. However, 27% of the isolates were susceptible to all tested antimicrobials. More turkey and pork isolates were resistant to ampicillin, penicillin, and tetracycline than were beef isolates (P < 0.05). Additionally, 17% of the turkey and 17% of the pork isolates were resistant to methicillin (MIC >/= 16 mug/ml), whereas no beef isolates were resistant to the antimicrobial agent. A single MRSA (methicillin MIC > 32 mug/ml) isolate containing the mecA gene with additional resistance to erythromycin, clindamycin, oxacillin plus 2% NaCl, cefoxitin, ampicillin, penicillin, quinupristin-dalfopristin, tetracycline, and gentamicin was recovered from one pork sample. The presence of antimicrobial-resistant S. aureus, coupled with the relative lack of such studies in the United States, suggests that further investigations on MRSA in the food supply are needed despite the low rate of MRSA found in this particular study
AFFILIATIONS:  Gemstone Program at the University of Maryland, University of Maryland, College Park, Maryland 20742, USA