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U.S. Department of Health and Human Services

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TITLE:  A single-laboratory validated method for the generation of DNA barcodes for the identification of fish for regulatory compliance
 
AUTHORS:  Handy SM;Deeds JR;Ivanova NV;Hebert PD;Hanner RH;Ormos A;Weigt LA;Moore MM;Yancy HF;
 
YEAR:  2011
 
JOURNAL ABBREV:  J AOAC Int
 
MONTH:  Jan
 
TYPE:  JOUR
 
REFMAN INDEX:  620
 
JOURNAL FULL:  Journal of the AOAC International
 
VOLUME:  94
 
ISSUE:  1
 
START PAGE:  201
 
END PAGE:  210
 
KEYWORDS:  Animals;Base Sequence;classification;Dna;DNA BARCODES;DNA Barcoding,Taxonomic;DNA Primers;drug;FISH;Fishes;Food;Food Safety;Food Supply;GENE;genetics;IDENTIFICATION;ILLNESS;legislation & jurisprudence;methods;Nutrition;Pilot Projects;Polymerase Chain Reaction;Research;Safety;Seafood;SPECIES IDENTIFICATION;Species Specificity;standards;United States;United States Food and Drug Administration;VALIDATION;
 
ABSTRACT:  The U.S. Food and Drug Administration is responsible for ensuring that the nation's food supply is safe and accurately labeled. This task is particularly challenging in the case of seafood where a large variety of species are marketed, most of this commodity is imported, and processed product is difficult to identify using traditional morphological methods. Reliable species identification is critical for both foodborne illness investigations and for prevention of deceptive practices, such as those where species are intentionally mislabeled to circumvent import restrictions or for resale as species of higher value. New methods that allow accurate and rapid species identifications are needed, but any new methods to be used for regulatory compliance must be both standardized and adequately validated. 'DNA barcoding' is a process by which species discriminations are achieved through the use of short, standardized gene fragments. For animals, a fragment (655 base pairs starting near the 5' end) of the cytochrome c oxidase subunit 1 mitochondrial gene has been shown to provide reliable species level discrimination in most cases. We provide here a protocol with single-laboratory validation for the generation of DNA barcodes suitable for the identification of seafood products, specifically fish, in a manner that is suitable for FDA regulatory use
 
AFFILIATIONS:  U.S. Food and Drug Administration, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, College Park, MD 20740, USA. Sara.Handy@fda.hhs.gov
 
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