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U.S. Department of Health and Human Services

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TITLE:  Presence and Characterization of Shiga Toxin-Producing Escherichia coli and Other Potentially Diarrheagenic E. coli Strains in Retail Meats
 
AUTHORS:  Xia XD;Meng JH;McDermott PF;Ayers S;Blickenstaff K;Tran TT;Abbott J;Zheng J;Zhao SH;
 
YEAR:  2010
 
JOURNAL ABBREV:  Appl Environ Microbiol
 
MONTH:  Mar
 
TYPE:  JOUR
 
REFMAN INDEX:  590
 
JOURNAL FULL:  Applied and environmental microbiology
 
VOLUME:  76
 
ISSUE:  6
 
START PAGE:  1709
 
END PAGE:  1717
 
KEYWORDS:  analysis;antimicrobial;ANTIMICROBIAL RESISTANCE;CELLS;Dna;Escherichia coli;ESCHERICHIA-COLI;Food;GENE;GENES;GROUND-BEEF;Humans;INFECTION;INFECTIONS;INTESTINAL MUCUS;INTIMIN TYPES;LISTERIA-MONOCYTOGENES;Maryland;Meat;microbiology;Prevalence;retail meat;RETAIL MEATS;Safety;SAO-PAULO;SEROTYPES;Shiga Toxin;STRAIN;STRAINS;SYSTEM;Vero Cells;Virulence;VIRULENCE GENES;
 
ABSTRACT:  To determine the presence of Shiga toxin-producing Escherichia coli (STEC) and other potentially diarrheagenic E. coli strains in retail meats, 7,258 E. coli isolates collected by the U. S. National Antimicrobial Resistance Monitoring System (NARMS) retail meat program from 2002 to 2007 were screened for Shiga toxin genes. In addition, 1,275 of the E. coli isolates recovered in 2006 were examined for virulence genes specific for other diarrheagenic E. coli strains. Seventeen isolates (16 from ground beef and 1 from a pork chop) were positive for stx genes, including 5 positive for both stx(1) and stx(2), 2 positive for stx(1), and 10 positive for stx(2). The 17 STEC strains belonged to 10 serotypes: O83:H8, O8:H16, O15:H16, O15:H17, O88:H38, ONT:H51, ONT:H2, ONT:H10, ONT:H7, and ONT:H46. None of the STEC isolates contained eae, whereas seven carried enterohemorrhagic E. coli (EHEC) hlyA. All except one STEC isolate exhibited toxic effects on Vero cells. DNA sequence analysis showed that the stx(2) genes from five STEC isolates encoded mucus-activatable Stx2d. Subtyping of the 17 STEC isolates by pulsed-field gel electrophoresis (PFGE) yielded 14 distinct restriction patterns. Among the 1,275 isolates from 2006, 11 atypical enteropathogenic E. coli (EPEC) isolates were identified in addition to 3 STEC isolates. This study demonstrated that retail meats, mainly ground beef, were contaminated with diverse STEC strains. The presence of atypical EPEC strains in retail meat is also of concern due to their potential to cause human infections
 
AFFILIATIONS:  Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USAUniv Maryland, Joint Inst Food Safety & Appl Nutr, College Pk, MD 20742 USAUS FDA, Ctr Vet Med, Laurel, MD 20708 USAUS FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA
 
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