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TITLE:  Development and validation of a resistance and virulence gene microarray targeting Escherichia coli and Salmonella enterica
 
AUTHORS:  Davis MA;Lim JY;Soyer Y;Harbottle H;Chang YF;New D;Orfe LH;Besser TE;Call DR;
 
YEAR:  2010
 
MONTH:  Jul
 
TYPE:  JOUR
 
REFMAN INDEX:  587
 
JOURNAL FULL:  Journal of Microbiological Methods
 
VOLUME:  82
 
ISSUE:  1
 
START PAGE:  36
 
END PAGE:  41
 
KEYWORDS:  ANTIMICROBIAL RESISTANCE;DIFFERENTIALLY EXPRESSED GENES;DNA MICROARRAY;Enterobacteriaceae;Escherichia coli;ESCHERICHIA-COLI;Food;GENE;GENES;GENOME SEQUENCE;Laboratories;MEDIATED QUINOLONE RESISTANCE;Microarray;MOLECULAR CHARACTERIZATION;OLIGONUCLEOTIDE MICROARRAY;PLATFORM COMPARABILITY;QUALITY-CONTROL;Replicon;Resistance genes;Salmonella;Salmonella enterica;SPECTRUM BETA-LACTAMASES;VALIDATION;Virulence;VIRULENCE GENES;
 
ABSTRACT:  A microarray was developed to simultaneously screen Escherichia coli and Salmonella enterica for multiple genetic traits. The final array included 203 60-mer oligonucleotide probes, including 117 for resistance genes, 16 for virulence genes, 25 for replicon markers, and 45 other markers. Validity of the array was tested by assessing inter-laboratory agreement among four collaborating groups using a blinded study design. Internal validation indicated that the assay was reliable (area under the receiver-operator characteristic curve = 0.97). Inter-laboratory agreement, however, was poor when estimated using the intraclass correlation coefficient, which ranged from 0.27 (95% confidence interval 0.24, 0.29) to 0.29 (0.23, 0.34). These findings suggest that extensive testing and procedure standardization will be needed before bacterial genotyping arrays can be readily shared between laboratories. (C) 2010 Elsevier B.V. All rights reserved
 
AFFILIATIONS:  Washington State Univ, Dept Vet Microbiol & Pathol, Coll Vet Med, Pullman, WA 99164 USAUniv Idaho, Microbiol Mol Biol & Biochem Dept, Moscow, ID 83844 USACornell Univ, Dept Food Sci, Ithaca, NY 14853 USAUS FDA, Ctr Vet Med, Laurel, MD 20708 USACornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA
 
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