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U.S. Department of Health and Human Services

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TITLE:  Characterization of Extended-Spectrum Cephalosporin-Resistant Salmonella enterica Serovar Heidelberg Isolated from Humans in the United States
AUTHORS:  Folster JP;Pecic G;Bolcen S;Theobald L;Hise K;Carattoli A;Zhao SH;McDermott PF;Whichard JM;
YEAR:  2010
JOURNAL ABBREV:  Foodborne Pathog Dis
JOURNAL FULL:  Foodborne pathogens and disease
END PAGE:  187
KEYWORDS:  AMPC BETA-LACTAMASE;analysis;Animals;BETA-LACTAMASE;Ceftriaxone;Cephalosporin Resistance;control;ESCHERICHIA-COLI;Food;FRANCE;GENE;GENES;Humans;INFECTIONS;Meat;OUTBREAKS;PLASMID;Plasmids;Poultry;retail meat;RETAIL MEATS;Salmonella;Salmonella enterica;STRAIN;SUSCEPTIBILITY;United States;UNITED-STATES;
ABSTRACT:  During the past decade, extended-spectrum cephalosporin resistance has increased among human isolates of Salmonella enterica serovar Heidelberg, the fourth most common serotype in the United States. We therefore characterized 54 Heidelberg isolates with decreased susceptibility (minimum inhibitory concentrations >= 2 mg/L) to ceftriaxone or ceftiofur; 49 (90.7%) contained the CMY-type beta-lactamase (bla(CMY)) gene. The 49 bla(CMY)-positive human Heidelberg isolates demonstrated a high degree of relatedness; 4 clusters (25 isolates total) had indistinguishable XbaI and BlnI patterns by pulsed-field gel electrophoresis and were indistinguishable from 42 retail meat Heidelberg isolates. Further characterization of 15 of these isolates demonstrated that all of the bla genes were bla(CMY-2) and plasmid-encoded, and most (11/15) of the plasmids were approximately 100 kb in size and belong to the incompatibility group I1 (IncI1). All five IncI1 plasmids tested by plasmid multilocus sequence typing analysis were ST12. This report suggests that extended-spectrum cephalosporin resistance among human Heidelberg isolates is mediated by the spread of a common IncI1 bla(CMY-2) plasmid, which may have a preference for a particular genetic background
AFFILIATIONS:  Ctr Dis Control & Prevent, CCID, NCZVED, DFBMD,EDLB, Atlanta, GA 30329 USAAtlanta Res & Educ Fdn, Decatur, GA USAIst Super Sanita, Dept Infect Parasit & Immune Mediated Dis, I-00161 Rome, ItalyUS FDA, Div Anim & Food Microbiol, Res Off, Ctr Vet Med, Laurel, MD USA