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TITLE:  An enhanced discriminatory pulsed-field gel electrophoresis scheme for subtyping Salmonella serotypes Heidelberg, Kentucky, SaintPaul, and Hadar
 
AUTHORS:  Xi M;Zheng J;Zhao S;Brown EW;Meng J;
 
YEAR:  2008
 
JOURNAL ABBREV:  J Food Prot
 
MONTH:  Oct
 
TYPE:  JOUR
 
REFMAN INDEX:  497
 
JOURNAL FULL:  Journal of food protection
 
VOLUME:  71
 
ISSUE:  10
 
START PAGE:  2067
 
END PAGE:  2072
 
KEYWORDS:  analysis;Animals;Bacterial Typing Techniques;Chickens;China;classification;Colony Count,Microbial;Consumer Product Safety;Dna;DNA Restriction Enzymes;Electrophoresis,Gel,Pulsed-Field;Enzymes;Food;Food Contamination;Food Microbiology;isolation & purification;Meat;methods;microbiology;Phylogeny;Poultry;Research;Salmonella;Species Specificity;Swine;United States;
 
ABSTRACT:  Conventional pulsed-field gel electrophoresis (PFGE) protocols, used extensively as a successful approach for subtyping many salmonellae, may be inadequate for discriminating strains sharing levels of homogeneity within the same serotype. Four additional restriction enzymes (SpeI, PacI, SfiI, and NotI), in addition to XbaI and BlnI, were used in PFGE typing of 33 Salmonella Heidelberg, 27 Salmonella Kentucky, 27 Salmonella SaintPaul, and 27 Salmonella Hadar isolates that were recovered from poultry and porcine retail meats from different states of the United States. A dendrogram derived from the combined analysis of six enzymes was highly discriminatory with a Simpson index of diversity value of over 0.950. The ratio of nodes to isolates was more than 0.75 with an average of fewer than three isolates in each polytomy for all four serotypes. Two three-enzyme combinations, SpeI/NotI/SfiI for Salmonella Heidelberg and Salmonella Hadar, and SpeI/BlnI/SfiI for Salmonella Kentucky and Salmonella SaintPaul, were found to have comparable discriminatory abilities of differentiating isolates of these Salmonella serotypes with the six-enzyme combination. The enhanced discriminatory PFGE-based subtyping scheme can be used effectively for the differentiation of strains of the four Salmonella serotypes. The findings also highlight PFGE analysis as a continued essential and informative subtyping method for source tracking and outbreak investigations of these and other Salmonella pathogens
 
AFFILIATIONS:  College of Food Science and Engineering, Northwest A&F University, Shaanxi, China
 
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