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TITLE:  Characterization of Salmonella enterica serovar Heidelberg from turkey-associated sources
 
AUTHORS:  Kaldhone P;Nayak R;Lynne AM;David DE;McDermott PF;Logue CM;Foley SL;
 
YEAR:  2008
 
JOURNAL ABBREV:  Appl Environ Microbiol
 
MONTH:  Aug
 
TYPE:  JOUR
 
REFMAN INDEX:  311
 
JOURNAL FULL:  Applied and environmental microbiology
 
VOLUME:  74
 
ISSUE:  16
 
START PAGE:  5038
 
END PAGE:  5046
 
KEYWORDS:  analysis;Animals;Bacterial Typing Techniques;classification;Conjugation,Genetic;Digestion;drug effects;Drug Resistance,Multiple,Bacterial;Electrophoresis,Gel,Pulsed-Field;Environment;Food Handling;Food Microbiology;genetics;Kanamycin;Microbial Sensitivity Tests;microbiology;pharmacology;Plasmids;Research;Salmonella;Salmonella enterica;Salmonella Infections,Animal;Streptomycin;Tetracycline;Turkeys;veterinary;
 
ABSTRACT:  Salmonella enterica serovar Heidelberg strains are frequently associated with food-borne illness, with recent isolates showing higher rates of resistance to multiple antimicrobial agents. One hundred eighty S. enterica serovar Heidelberg isolates, collected from turkey-associated production and processing sources, were tested for antimicrobial susceptibility and compared by pulsed-field gel electrophoresis (PFGE) and plasmid profile analysis. The potential for the transfer of resistance between strains was studied by conjugation experiments. PFGE analysis using XbaI digestion identified eight clusters (based on 90% similarity), with the largest containing 71% of the isolates. Forty-two percent of the isolates were resistant to at least 1 of the 15 antimicrobial agents tested, and 4% of the isolates were resistant to 8 or more antimicrobial agents. Resistances to streptomycin (32%), tetracycline (30%), and kanamycin (24%) were most commonly detected. Interestingly, the XbaI PFGE profiles of selective multidrug-resistant strains (n = 22) of S. enterica serovar Heidelberg from turkey-associated sources were indistinguishable from the predominant profile (JF6X01.0022) detected in isolates associated with human infections. These isolates were further differentiated into seven distinct profiles following digestion with the BlnI enzyme, with the largest cluster comprising 15 isolates from veterinary diagnostic and turkey processing environments. Conjugation experiments indicated that resistance to multiple antimicrobial agents was transferable among strains with diverse PFGE profiles
 
AFFILIATIONS:  Marshfield Clinic Research Foundation, 1000 North Oak Avenue, Marshfield, WI 54449, USA
 
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