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U.S. Department of Health and Human Services

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TITLE:  Quinupristin-dalfopristin resistance in Enterococcus faecium isolates from humans, farm animals, and grocery store meat in the United States
 
AUTHORS:  Donabedian SM;Perri MB;Vager D;Hershberger E;Malani P;Simjee S;Chow J;Vergis EN;Muder RR;Gay K;Angulo FJ;Bartlett P;Zervos MJ;
 
YEAR:  2006
 
JOURNAL ABBREV:  J Clin Microbiol
 
MONTH:  Sep
 
TYPE:  JOUR
 
REFMAN INDEX:  500
 
JOURNAL FULL:  Journal of clinical microbiology
 
VOLUME:  44
 
ISSUE:  9
 
START PAGE:  3361
 
END PAGE:  3365
 
KEYWORDS:  Animals;Animals,Domestic;Anti-Bacterial Agents;Bacterial Proteins;Cattle;Chickens;drug effects;Drug Resistance,Bacterial;Electrophoresis,Gel,Pulsed-Field;Enterococcus;Enterococcus faecium;genetics;Gram-Positive Bacterial Infections;Humans;isolation & purification;Meat;microbiology;pharmacology;Proteins;Research;Swine;Turkeys;United States;Virginiamycin;
 
ABSTRACT:  Three hundred sixty-one quinupristin-dalfopristin (Q-D)-resistant Enterococcus faecium (QDREF) isolates were isolated from humans, turkeys, chickens, swine, dairy and beef cattle from farms, chicken carcasses, and ground pork from grocery stores in the United States from 1995 to 2003. These isolates were evaluated by pulsed-field gel electrophoresis (PFGE) to determine possible commonality between QDREF isolates from human and animal sources. PCR was performed to detect the streptogramin resistance genes vatD, vatE, and vgbA and the macrolide resistance gene ermB to determine the genetic mechanism of resistance in these isolates. QDREF from humans did not have PFGE patterns similar to those from animal sources. vatE was found in 35%, 26%, and 2% of QDREF isolates from turkeys, chickens, and humans, respectively, and was not found in QDREF isolates from other sources. ermB was commonly found in QDREF isolates from all sources. Known streptogramin resistance genes were absent in the majority of isolates, suggesting the presence of other, as-yet-undetermined, mechanisms of Q-D resistance
 
AFFILIATIONS:  Henry Ford Hospital, Detroit, MI, USA
 
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