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TITLE:  Application of molecular techniques to the study of hospital infection
 
AUTHORS:  Singh A;Goering RV;Simjee S;Foley SL;Zervos MJ;
 
YEAR:  2006
 
JOURNAL ABBREV:  Clin Microbiol Rev
 
MONTH:  Jul
 
TYPE:  JOUR
 
REFMAN INDEX:  278
 
JOURNAL FULL:  Clinical microbiology reviews
 
VOLUME:  19
 
ISSUE:  3
 
START PAGE:  512
 
END PAGE:  530
 
KEYWORDS:  analysis;Bacteria;Bacterial Infections;Bacterial Typing Techniques;Base Sequence;classification;Cross Infection;Dna;economics;epidemiology;Epidemiology,Molecular;Genetic Techniques;genetics;Genotype;Humans;isolation & purification;Laboratories;methods;microbiology;Molecular Sequence Data;mortality;Phenotype;Polymerase Chain Reaction;prevention & control;Research;United States;
 
ABSTRACT:  Nosocomial infections are an important source of morbidity and mortality in hospital settings, afflicting an estimated 2 million patients in United States each year. This number represents up to 5% of hospitalized patients and results in an estimated 88,000 deaths and 4.5 billion dollars in excess health care costs. Increasingly, hospital-acquired infections with multidrug-resistant pathogens represent a major problem in patients. Understanding pathogen relatedness is essential for determining the epidemiology of nosocomial infections and aiding in the design of rational pathogen control methods. The role of pathogen typing is to determine whether epidemiologically related isolates are also genetically related. To determine molecular relatedness of isolates for epidemiologic investigation, new technologies based on DNA, or molecular analysis, are methods of choice. These DNA-based molecular methodologies include pulsed-field gel electrophoresis (PFGE), PCR-based typing methods, and multilocus sequence analysis. Establishing clonality of pathogens can aid in the identification of the source (environmental or personnel) of organisms, distinguish infectious from noninfectious strains, and distinguish relapse from reinfection. The integration of molecular typing with conventional hospital epidemiologic surveillance has been proven to be cost-effective due to the associated reduction in the number of nosocomial infections. Cost-effectiveness is maximized through the collaboration of the laboratory, through epidemiologic typing, and the infection control department during epidemiologic investigations
 
AFFILIATIONS:  Department of Medicine, Section of Infectious Diseases, Henry Ford Hospital, Wayne State University School of Medicine, Detroit, MI 48202, USA
 
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