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TITLE:  Role of efflux pumps and topoisomerase mutations in fluoroquinolone resistance in Campylobacter jejuni and Campylobacter coli
 
AUTHORS:  Ge B;McDermott PF;White DG;Meng J;
 
YEAR:  2005
 
JOURNAL ABBREV:  Antimicrob Agents Chemother
 
MONTH:  Aug
 
TYPE:  JOUR
 
REFMAN INDEX:  212
 
JOURNAL FULL:  Antimicrobial agents and chemotherapy
 
VOLUME:  49
 
ISSUE:  8
 
START PAGE:  3347
 
END PAGE:  3354
 
KEYWORDS:  analysis;Anti-Infective Agents;Bacterial Proteins;Campylobacter;Campylobacter coli;Campylobacter jejuni;Chloramphenicol;Ciprofloxacin;Dna;DNA Gyrase;drug effects;Drug Resistance,Bacterial;enzymology;Fluoroquinolones;Food;genetics;Humans;Maryland;Membrane Transport Proteins;metabolism;Microbial Sensitivity Tests;Molecular Sequence Data;Mutagenesis,Insertional;Mutation;Nalidixic Acid;pharmacology;Point Mutation;Proteins;Research;Sequence Analysis,DNA;Tetracycline;
 
ABSTRACT:  Point mutations in the topoisomerase (DNA gyrase A) gene are known to be associated with fluoroquinolone resistance in Campylobacter. Recent studies have shown that an efflux pump encoded by cmeABC is also involved in decreased susceptibilities to fluoroquinolones, as well as other antimicrobials. Genome analysis suggests that Campylobacter jejuni contains at least nine other putative efflux pumps. Using insertional inactivation and site-directed mutagenesis, we investigated the potential contributions of these pumps to susceptibilities to chloramphenicol, ciprofloxacin, erythromycin, and tetracycline in C. jejuni and Campylobacter coli. Insertional inactivation of cmeB resulted in 4- to 256-fold decreases in the MICs of chloramphenicol, ciprofloxacin, erythromycin, and tetracycline, with erythromycin being the most significantly affected. In contrast, inactivation of all other putative efflux pumps had no effect on susceptibility to any of the four antimicrobials tested. Mutation of gyrA at codon 86 (Thr-Ile) caused 128- and 64-fold increases in the MICs of ciprofloxacin and nalidixic acid, respectively. The replacement of the mutated gyrA with a wild-type gyrA allele resulted in a 32-fold decrease in the ciprofloxacin MIC and no change in the nalidixic acid MIC. Our findings indicate that CmeABC is the only efflux pump among those tested that influences antimicrobial resistance in Campylobacter and that a point mutation (Thr-86-Ile) in gyrA directly causes fluoroquinolone resistance in Campylobacter. These two mechanisms work synergistically in acquiring and maintaining fluoroquinolone resistance in Campylobacter species
 
AFFILIATIONS:  Department of Nutrition and Food Science, 0112 Skinner Building, University of Maryland, College Park, MD 20742, USA
 
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