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TITLE:  Antimicrobial resistance: standardisation and harmonisation of laboratory methodologies for the detection and quantification of antimicrobial resistance
 
AUTHORS:  White DG;Acar J;Anthony F;Franklin A;Gupta R;Nicholls T;Tamura Y;Thompson S;Threlfall EJ;Vose D;van VM;Wegener HC;Costarrica ML;
 
YEAR:  2001
 
JOURNAL ABBREV:  Rev Sci Tech
 
MONTH:  Dec
 
TYPE:  JOUR
 
REFMAN INDEX:  252
 
JOURNAL FULL:  Revue scientifique et technique (International Office of Epizootics)
 
VOLUME:  20
 
ISSUE:  3
 
START PAGE:  849
 
END PAGE:  858
 
KEYWORDS:  Agar;Animals;Bacteria;Diffusion;Drug Resistance,Bacterial;Food;Humans;International Cooperation;Laboratories;Maryland;methods;Microbial Sensitivity Tests;Quality Control;Reference Standards;Research;standards;trends;veterinary;Veterinary Medicine;
 
ABSTRACT:  The Ad hoc Group of experts on antimicrobial resistance of the Office International des Epizooties has developed a guideline on the standardisation and harmonisation of laboratory methodologies used for the detection and quantification of antimicrobial resistance. The existing methods (disk diffusion [including concentration gradient strips], agar dilution and broth dilution) are reviewed, including a comparison of their advantages and disadvantages. The definitions of resistance characteristics of bacteria (susceptible, intermediate and resistant) are addressed and the criteria for the establishment of breakpoints are discussed. Due consideration has to be given to these aspects in the interpretation and comparison of resistance monitoring or surveillance data. The use of validated laboratory methods and the establishment of quality assurance (internal and external) for microbiological laboratory work and the reporting of quantitative test results is recommended. Equivalence of different methods and laboratory test results is also recommended to be established by external proficiency testing, which should be achieved by the means of a reference laboratory system. This approach allows the comparison of test results obtained using different methods generated by laboratories in different countries
 
AFFILIATIONS:  Centre for Veterinary Medicine, Food and Drug Administration, Office of Research, HFV-530, 8401 Muirkirk Road, Laurel, Maryland 20708, USA
 
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