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TITLE:  Validation of a polymerase chain reaction method for the detection of rendered bovine-derived materials in feedstuffs
 
AUTHORS:  Myers MJ;Friedman SL;Farrell DE;Dove-Pettit DA;Bucker MF;Kelly S;Madzo S;Campbell W;Wang RF;Paine D;Cerniglia CE;
 
YEAR:  2001
 
JOURNAL ABBREV:  J Food Prot
 
MONTH:  Apr
 
TYPE:  JOUR
 
REFMAN INDEX:  60
 
JOURNAL FULL:  Journal of food protection
 
VOLUME:  64
 
ISSUE:  4
 
START PAGE:  564
 
END PAGE:  566
 
KEYWORDS:  analysis;Animal Feed;Animals;Cattle;False Negative Reactions;False Positive Reactions;Food;genetics;Laboratories;Maryland;Meat;methods;Polymerase Chain Reaction;Reproducibility of Results;Research;Sensitivity and Specificity;veterinary;Veterinary Medicine;
 
ABSTRACT:  This study validated a polymerase chain reaction-based method for the detection of a specific bovine mitochondrial gene derived from rendered bovine tissues and admixed with complete animal feed. Four laboratories participated in this effort: one state laboratory and three Food and Drug Administration (FDA) laboratories, including one FDA field laboratory. The protocol used a statistical approach of 90% probability, with a 95% confidence interval for determining acceptable rates of false-positive and false-negative samples. Each participating laboratory analyzed 30 samples of feed each containing 0, 0.125, and 2.0% bovine meat and bone meal (BMBM), for a total of 90 feed samples. The samples were randomized such that the analysts were unaware of the true identity of the test samples. The results demonstrated that all laboratories met the acceptance criteria established for this protocol. The overall rates of false-negative results were 0.83% (1/120) at the level of 0.125% BMBM and 1.67% (2/120) at the level of 2% BMBM. The overall rate of false-negative results for all levels of BMBM was 1.25% (3/240). The rate for false-positive results was 0.83%
 
AFFILIATIONS:  Division of Animal Research, Office of Research, Center for Veterinary Medicine, Food and Drug Administration, Laurel, Maryland 20708, USA. mmyers@cvm.fda.gov
 
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