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TITLE:  A rapid method for PCR detection of bovine materials in animal feedstuffs
 
AUTHORS:  Wang RF;Myers MJ;Campbell W;Cao WW;Paine D;Cerniglia CE;
 
YEAR:  2000
 
JOURNAL ABBREV:  Mol Cell Probes
 
MONTH:  Feb
 
TYPE:  JOUR
 
REFMAN INDEX:  61
 
JOURNAL FULL:  Molecular and cellular probes
 
VOLUME:  14
 
ISSUE:  1
 
START PAGE:  1
 
END PAGE:  5
 
KEYWORDS:  analysis;Animal Feed;Animals;Biological Products;Cattle;Dna;DNA Primers;Encephalopathy,Bovine Spongiform;genetics;instrumentation;Meat;methods;microbiology;Minerals;Polymerase Chain Reaction;prevention & control;Research;Sensitivity and Specificity;standards;Time Factors;transmission;
 
ABSTRACT:  Rapid identification of bovine materials in animal feedstuffs is essential for effective control of a potential source of bovine spongiform encephalopathy. We have developed a rapid method for the detection of the presence of bovine materials in animal feeds. Animal feed samples were prepared by a Chelex-100 treatment method, then subjected to polymerase chain reaction (PCR) detection. The assay can be completed in 2 h including 30 min for sample preparation, 35-65 min for PCR cycling and 30 min for gel electrophoresis. This method is not only rapid, simple and consistent, but also avoids a hazardous waste disposal issue associated with a previously described guanidine thiocyanate (GuSCN) extraction-PCR method
 
AFFILIATIONS:  Microbiology Division, National Center for Toxicological Research, Jefferson, AR 72079, USA. rwang@nctr.fda.gov
 
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