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TITLE:  Confirmation of multiple tetracycline residues in milk and oxytetracycline in shrimp by liquid chromatography-particle beam mass spectrometry
 
AUTHORS:  Carson MC;Ngoh MA;Hadley SW;
 
YEAR:  1998
 
JOURNAL ABBREV:  J Chromatogr B Biomed Sci Appl
 
MONTH:  Aug
 
TYPE:  JOUR
 
REFMAN INDEX:  139
 
JOURNAL FULL:  Journal of chromatography B, Biomedical sciences and applications
 
VOLUME:  712
 
ISSUE:  40910
 
START PAGE:  113
 
END PAGE:  128
 
KEYWORDS:  analysis;Animals;Anti-Bacterial Agents;chemistry;Chromatography;Chromatography,Liquid;Decapoda (Crustacea);Drug Residues;Food;Mass Spectrometry;Milk;Oxytetracycline;Sensitivity and Specificity;Shellfish;Solid Phase Extraction;Tetracycline;Tetracyclines;veterinary;Veterinary Medicine;
 
ABSTRACT:  A confirmation procedure is described for detection of residues of six tetracyclines in bovine milk, and oxytetracycline in shrimp. Residues are extracted from milk or shrimp tissue using metal chelate affinity chromatography. The extracts are desalted, further concentrated using polymeric solid-phase extraction, and chromatographed on a polymeric reversed-phase column. Analysis is by methane negative ion chemical ionization on a quadrupole mass spectrometer using a particle beam interface. Data are acquired in partial scan mode, monitoring from m/z 378 to m/z 480. The procedure was validated with control milk and shrimp, fortified milk (30 ng/ml) and shrimp (100 ng/g), and milk and tissue from animals treated with the drugs
 
AFFILIATIONS:  US Food and Drug Administration, Center for Veterinary Medicine, Office of Research, Laurel, MD 20708, USA
 
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