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TITLE:  Confirmation of spectinomycin in milk using ion-pair solid-phase extraction and liquid chromatography-electrospray ion trap mass spectrometry
 
AUTHORS:  Carson MC;Heller DN;
 
YEAR:  1998
 
JOURNAL ABBREV:  J Chromatogr B Biomed Sci Appl
 
MONTH:  Oct
 
TYPE:  JOUR
 
REFMAN INDEX:  21
 
JOURNAL FULL:  Journal of chromatography B, Biomedical sciences and applications
 
VOLUME:  718
 
ISSUE:  1
 
START PAGE:  95
 
END PAGE:  102
 
KEYWORDS:  analysis;Animals;Anti-Bacterial Agents;Cattle;chemistry;Chromatography,Liquid;Electrochemistry;Female;Food;Ions;Mass Spectrometry;methods;Milk;Solid Phase Extraction;Spectinomycin;veterinary;Veterinary Medicine;
 
ABSTRACT:  A confirmation procedure is described for residues of spectinomycin in bovine milk. Spectinomycin is extracted from raw milk using ion-pair reversed-phase solid-phase extraction. The extracts are ion-pair chromatographed on a polymeric reversed-phase column and analyzed on a quadrupole ion trap mass spectrometer equipped with an electrospray interface. MS-MS data are acquired in the scan mode of product ions deriving from m/z 333, the protonated molecular ion. The estimated limit of confirmation is between 0.05 and 0.1 microg/ml. The procedure was validated with control milk, fortified milk (0.1-5.0 microg/ml), and milk from cows dosed with spectinomycin
 
AFFILIATIONS:  Center for Veterinary Medicine, US Food and Drug Administration, Laurel, MD 20708, USA
 
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