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TITLE:  Determination of tilmicosin in bovine milk by liquid chromatography with ultraviolet detection
 
AUTHORS:  Ngoh MA;
 
YEAR:  1996
 
JOURNAL ABBREV:  J AOAC Int
 
MONTH:  May
 
TYPE:  JOUR
 
REFMAN INDEX:  288
 
JOURNAL FULL:  Journal of AOAC International
 
VOLUME:  79
 
ISSUE:  3
 
START PAGE:  652
 
END PAGE:  655
 
KEYWORDS:  analogs & derivatives;analysis;Animals;Anti-Bacterial Agents;Cattle;Centrifugation;chemistry;Chromatography;Chromatography,Liquid;Female;Food;Macrolides;Milk;Solid Phase Extraction;Tylosin;Ultraviolet Rays;veterinary;Veterinary Medicine;
 
ABSTRACT:  A method is described for determining tilmicosin in bovine milk by liquid chromatography with ultraviolet (UV) detection. Samples are defatted by centrifugation. The lower skim layer is cleaned on a C18 solid-phase extraction cartridge. The extract is concentrated and analyzed with a reversed-phase phenyl column with UV detection at 280 nm. The method was validated with control milk fortified at 50, 100, and 200 ng/mL. Average recoveries (and intralaboratory coefficients of variation) were 97% (9%), 98% (5%), and 101% (3%), respectively. The limit of quantitation is approximately 20 ng/mL, and the limit of detection is approximately 13 ng/mL. The method was tested on milk from a cow dosed with tilmicosin
 
AFFILIATIONS:  U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Science, Beltsville, MD 20705, USA
 
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