• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services

CVM Publications - Detail

  • Print
  • Share
  • E-mail
-
 
TITLE:  Development and Validation of A Liquid-Chromatographic Method for the Determination of Furosemide, A Diuretic, in Bovine-Milk
 
AUTHORS:  Shaikh B;
 
YEAR:  1995
 
JOURNAL ABBREV:  J Agric Food Chem
 
MONTH:  Aug
 
TYPE:  JOUR
 
REFMAN INDEX:  561
 
JOURNAL FULL:  Journal of agricultural and food chemistry
 
VOLUME:  43
 
ISSUE:  8
 
START PAGE:  2117
 
END PAGE:  2121
 
KEYWORDS:  bovine milk;BOVINE-MILK;Centrifugation;DIURETIC;Fluorescence;FLUORESCENCE DETECTION;Furosemide;HPLC;incurred residue;LC;Milk;PLASMA;REVERSED PHASE;Temperature;urine;VALIDATION;
 
ABSTRACT:  A rapid and sensitive reversed phase liquid chromatographic (LC) procedure was developed and validated for the quantitation of furosemide, a diuretic, in bovine milk. Whole milk was defatted by initial centrifugation at room temperature. The resulting skim milk was deproteinated with acetonitrile and centrifuged again. The acetonitrile from the supernatant was evaporated, and the remaining aqueous portion was directly analyzed by LC. The LC conditions employed include a Spherisorb 5 ODS 2 column, a phosphate/acetonitrile buffer (pH 3), and a fluorescence detector set at 272 and 410 nm excitation and emission wavelengths, respectively. The average recoveries of furosemide from milk fortified at 5, 10, and 20 ppb were 108, 91, and 85%, respectively, with corresponding CVs of 14, 8, and 6%. The method was validated by assaying milk obtained from a cow dosed intravenously with 500 mg of furosemide. The furosemide concentrations in 8 and 24 h milk samples were determined to be about 150 and 5 ppb, respectively. No furosemide was detected in 32 and 48 h samples
 
-
-