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TITLE:  Confirmation of leucogentian violet in chicken fat by gas chromatography/mass spectrometry
 
AUTHORS:  Wilson RT;Wong J;Johnston J;Epstein R;Heller DN;
 
YEAR:  1994
 
JOURNAL ABBREV:  J AOAC Int
 
MONTH:  Sep
 
TYPE:  JOUR
 
REFMAN INDEX:  24
 
JOURNAL FULL:  Journal of AOAC International
 
VOLUME:  77
 
ISSUE:  5
 
START PAGE:  1137
 
END PAGE:  1142
 
KEYWORDS:  Adipose Tissue;analogs & derivatives;analysis;Animals;chemistry;Chickens;Food;Gas Chromatography-Mass Spectrometry;Gentian Violet;Ions;Laboratories;methods;Safety;statistics & numerical data;
 
ABSTRACT:  A gas chromatographic/mass spectrometric (GC/MS) procedure for confirming the identity of leucogentian violet (LGV) in chicken fat was developed for regulatory application. The unused portion of the extract remaining from a determinative procedure was back-extracted into an organic phase, concentrated, and analyzed by GC/MS. Confirmation of the identity of LGV was based on matching the retention times and relative abundances of 6 ions in the extract to corresponding values obtained for the LGV standard. The procedure was validated by replicate analyses of negative control, fortified control, and residue-incurred chicken fat. The presence of LGV was confirmed by the GC/MS procedure in all samples found to contain LGV by prior liquid chromatographic analyses. There were no interferences in the control samples
 
AFFILIATIONS:  U.S. Department of Agriculture, Food Safety and Inspection Service, Midwestern Laboratory, St. Louis, MO 63115
 
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