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U.S. Department of Health and Human Services

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TITLE:  Determination of Leucogentian Violet in Chicken Fat by Liquid-Chromatography with Uv-Detection
 
AUTHORS:  Heller DN;
 
YEAR:  1992
 
JOURNAL ABBREV:  J AOAC Int
 
MONTH:  Jul
 
TYPE:  JOUR
 
REFMAN INDEX:  536
 
JOURNAL FULL:  Journal of AOAC International
 
VOLUME:  75
 
ISSUE:  4
 
START PAGE:  650
 
END PAGE:  654
 
KEYWORDS:  Chickens;Chromatography;DEMETHYLATED METABOLITES;ELECTROCHEMICAL DETECTION;Gentian Violet;GENTIAN-VIOLET;
 
ABSTRACT:  UV detection is proposed as an alternative to electrochemical detection for the determination of leucogentian violet (LGV) in chicken fat by liquid chromatography. UV detection was combined with a previously reported extraction technique and new chromatographic conditions to yield a method that was evaluated at the target level of 10 ppb. Baseline separation of LGV from matrix interferences was achieved with a C18 bonded silica column and a mobile phase of methanol-pH 4 ammonium acetate (90 + 10). Average recovery for samples fortified at 5, 10, and 20 ppb was 76.4%, with a coefficient of variation (CV) of 4.8%. Fat from chickens raised on feed medicated with 30 ppm gentian violet was found to contain 17.6 ppb LGV, with a CV of 7.9%, after a 3-day withdrawal period. The detection limit for the system used was estimated to be 0.4 ng, and the limit of quantitation for the method was estimated to be 2 ppb
 
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