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TITLE:  Californium-252 plasma desorption mass spectrometry of aminoglycoside antibiotics
 
AUTHORS:  Khan AH;Shaikh B;Allen EH;Sokoloski EA;
 
YEAR:  1988
 
JOURNAL ABBREV:  Biomed Environ Mass Spectrom
 
MONTH:  Oct
 
TYPE:  JOUR
 
REFMAN INDEX:  446
 
JOURNAL FULL:  Biomedical & environmental mass spectrometry
 
VOLUME:  17
 
ISSUE:  4
 
START PAGE:  329
 
END PAGE:  335
 
KEYWORDS:  Aminoglycosides;analysis;Anti-Bacterial Agents;Californium;Drug Residues;Food;Gentamicins;Ions;Kanamycin;Maryland;Mass Spectrometry;methods;Neomycin;Streptomycin;Tobramycin;
 
ABSTRACT:  This paper presents mass spectral data of eleven aminoglycoside antibiotics by using californium-252 plasma desorption (252Cf PD) mass spectrometry. This mass spectral data could be used to develop a confirmatory method for monitoring aminoglycoside antibiotic residues isolated from food products of animal origin. Mass spectra were determined by applying time-of-flight 252Cf PD mass spectrometry to eleven aminoglycoside antibiotics, namely: neomycin, kanamycin, paromomycin, tobramycin, apramycin, streptomycin, dihydrostreptomycin, amikacin, netilmicin, sisomicin and gentamicins. All eleven antibiotics yielded positive ion spectra. These hydrophilic antibiotics were derivitized to extractable chromopheric compounds. All but two antibiotics (streptomycin and dihydrostreptomycin) yielded nitrophenyl derivatives and spectra were obtained in both negative and positive ion modes. Derivatized aminoglycosides produced cation and anion spectra with quasimolecular ions corresponding to [M + H]+., M+, [M - H]-., [M + Na]+, [M + K]+ and M-. or [M - H]- and M-. or [M - H]-. Underivatized antibiotics were best examined in the positive ion mode. 252Cf PD mass spectrometry consistently produced very strong molecular or quasimolecular ions for all aminoglycoside antibiotics
 
AFFILIATIONS:  Division of Production Drugs, US Food and Drug Administration, Rockville, Maryland 20857
 
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