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TITLE:  Reducing the effect of chemiluminescence on the efficiency of extracting 14C-neomycin from fortified kidney tissue as measured by a liquid scintillation system
 
AUTHORS:  Gridley JC;Allen EH;Shaikh B;
 
YEAR:  1986
 
JOURNAL ABBREV:  Int J Rad Appl Instrum B
 
TYPE:  JOUR
 
REFMAN INDEX:  88
 
JOURNAL FULL:  International journal of radiation applications and instrumentation Part B, Nuclear medicine and biology
 
VOLUME:  13
 
ISSUE:  1
 
START PAGE:  57
 
END PAGE:  61
 
KEYWORDS:  analysis;Animals;Carbon Radioisotopes;isolation & purification;Kidney;Luminescent Measurements;methods;Neomycin;Scintillation Counting;Solubility;
 
ABSTRACT:  Extraction efficiency was monitored, using 14C-neomycin and liquid scintillation counting, during development of a procedure to isolate neomycin from fortified kidney tissue. Chemiluminescence caused 119% false positive recovery at 0.16 ppm neomycin (1260 disintegrations/min (dpm) activity). However, the contribution of chemiluminescence to false positive recovery was negligible above 8 ppm neomycin (63,000 dpm activity). To reduce chemiluminescence, fortified kidney tissue was extracted with saline by homogenization, mixed with Protosol at 70 degrees C for 1 h, cooled 5 min at 4 degrees C and neutralized with acetic acid. The digest was mixed with Aquasol and incubated 1 h at 47 degrees C, followed by equilibration for 2 h in the counter. The procedure allows reliable monitoring of extraction efficiency down to about 407 dpm activity from 14C-neomycin (0.05 ppm) with maximum chemiluminescence of about 4%
 
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