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TITLE:  Quantitative thin layer chromatographic multi-sulfonamide screening procedure
 
AUTHORS:  Thomas MH;Soroka KE;Thomas SH;
 
YEAR:  1983
 
JOURNAL ABBREV:  J Assoc Off Anal Chem
 
MONTH:  Jul
 
TYPE:  JOUR
 
REFMAN INDEX:  143
 
JOURNAL FULL:  Journal - Association of Official Analytical Chemists
 
VOLUME:  66
 
ISSUE:  4
 
START PAGE:  881
 
END PAGE:  883
 
KEYWORDS:  analysis;Animals;Cattle;Chromatography;Chromatography,Thin Layer;Ducks;Liver;Meat;Methanol;Microchemistry;Muscles;Sulfadimethoxine;Sulfamethazine;Sulfaquinoxaline;Sulfonamides;Swine;Turkeys;
 
ABSTRACT:  In-situ optical scanning of fluorescamine derivatives on thin layer silica gel plates provides a rapid method for the determination of multiple sulfonamides at levels below 0.1 ppm. Sample preparation is minimal. Homogenized liver or muscle is extracted with ethyl acetate and then back-extracted into 0.2M glycine buffer. After pH adjustment, the extract is washed with hexane and extracted with methylene chloride. The organic phase is evaporated to dryness and reconstituted in methanol. Pre-adsorbent layer silica gel plates are used for chromatography. The method has been applied to residues of sulfamethazine, sulfadimethoxine, sulfathiazole, sulfaquinoxaline, and sulfabromomethazine in cattle, swine, turkey, and duck tissues
 
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