• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services

The Paper Trail - Detail

  • Print
  • Share
  • E-mail
-
 
Article, Chapter, or Book Title:  Interlaboratory evaluation of a real-time multiplex polymerase chain reaction method for identification of salmon and trout species in commercial products
 
Author(s):  Rasmussen Hellberg RS, Naaum AM, Handy SM, Hanner RH, Deeds JR, Yancy HF, Morrissey MT
 
Year:  2011
 
Journal Abbrev, Book Title, or Conference:  Journal of Agriculture and Food Chemistry
 
Journal Full Name:  Journal of Agriculture and Food Chemistry
 
Issue:  3
 
Volume:  59
 
Start Page:  876
 
End Page:  884
 
Key Words:  real-time PCR, interlaboratory study, salmon, trout, species identification, multiplex PCR
 
Abstract:  This interlaboratory study evaluated a real-time multiplex polymerase chain reaction (PCR) method for identification of salmon and trout species in a range of commercial products in North America. Eighty salmon and trout products were tested with this method by three independent laboratories. Samples were collected in the United States and Canada, and only the collecting institution was aware of the species declaration. Following analysis with real-time PCR, all three laboratories were able to identify species in 79 of the 80 products, with 100% agreement on species assignment. A low level of fraud was detected, with only four products (5%) found to be substituted or mixtures of two species. The results for two of the fraudulent products were confirmed with alternate methods, but the other two products were heavily processed and could not be verified with methods other than real-time PCR. Overall, the results of this study show the usefulness and versatility of this real-time PCR method for the identification of commercial salmon and trout species.
 
-
-