Scientific Publications by FDA Staff
Mol Microbiol 2005 Apr;56(1):175-88
BvgA functions as both an activator and a repressor to control Bvg phase expression of bipA in Bordetella pertussis.
Williams CL, Boucher PE, Stibitz S, Cotter PA
Cotter PA, Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA US FDA, Ctr Biol Evaluat & Res, Div Bacterial Parasit & Allergen Prod, Bethesda, MD 20892 USA
The Bordetella bipA gene is expressed maximally when the BvgAS phosphorelay is semi-active, i.e. in the Bvg-intermediate (Bvg(i)) phase. We used a BvgA-FeBABE cleavage approach together with site-directed mutagenesis and bipA-lacZ fusion analyses to determine precisely where BvgA-phosphate (BvgA approximately P) binds at the bipA promoter and how that binding contributes to the complex transcription pattern displayed by bipA. BvgA approximately P bound with high affinity and cooperatively with RNAP to sequences at the bipA promoter immediately 5' to and overlapping those bound by RNAP to activate transcription under Bvg(i) phase conditions. bipA therefore, like fhaB, appears to be similar to classical class-II promoters with regard to the mechanism by which its transcription is activated. BvgA approximately P bound with relatively low affinity to sequences immediately 3' of those bound by RNAP at the bipA promoter and this binding mediated repression of bipA transcription under Bvg+ phase conditions. BvgA approximately P binding to these sequences occurred simultaneously, if not cooperatively, with RNAP, indicating that BvgA approximately P represses bipA expression by inhibiting transcription initiation and/or elongation, rather than by competing with RNAP for binding. As bipA is the first Bvg(i) phase gene to be characterized, and the first gene shown to be repressed by BvgA approximately P directly, our results will provide a basis for comparison as additional Bvg-regulated genes are identified and characterized.
|Category: Journal Article|
|PubMed ID: #15773988|
|Includes FDA Authors from Scientific Area(s): Biologics|
|Entry Created: 2011-10-04||Entry Last Modified: 2012-08-29|