Scientific Publications by FDA Staff
Mol Biol Cell 2007 Sep;18(9):3290-301
Centrin1 Is Required for Organelle Segregation and Cytokinesis in Trypanosoma brucei.
Selvapandiyan A, Kumar P, Morris JC, Salisbury JL, Wang CC, Nakhasi HL
Selvapandiyan A (reprint author), US FDA, Ctr Biol Evaluat & Res, Div Emerging & Transfus Transmitted Dis, Bethesda, MD 20892 USA US FDA, Ctr Biol Evaluat & Res, Div Emerging & Transfus Transmitted Dis, Bethesda, MD 20892 USA Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA Clemson Univ, Dept Biochem & Genet, Clemson, SC 29634 USA Mayo Clin, Coll Med, Tumor Biol Program, Rochester, MN 55905 USA
Monitoring Editor: Orna Cohen-Fix Centrin is a calcium binding centrosome/basal body associated protein involved in duplication and segregation of these organelles in eukaryotes. We had shown that disruption of one of the centrin genes (centrin1) in Leishmania amastigotes resulted in failure of both basal body duplication and cytokinesis. Here, we undertook to define the role of centrin1 (TbCen1) in the duplication and segregation of basal body and its associated organelles kinetoplast and Golgi, as well as its role in cytokinesis of the procyclic form of Trypanosoma brucei by depleting its protein using RNAi methodology. TbCen1 depleted cells showed significant reduction in growth compared with control cells. Morphological analysis of these cells showed they were large and pleomorphic with multiple detached flagella. Both immunofluorescence assays using organelle specific antibodies and electron microscopic analysis showed that TbCen1 deficient cells contained multiple basal bodies, kinetoplasts, Golgi and nuclei. These multiple organelles were, however, closely clustered together, indicating duplication without segregation in the absence of centrin. This failure in organelle segregation may be the likely cause of inhibition of cytokinesis, suggesting for the first time a new and unique role for centrin in the segregation of organelles without affecting their multiplication in the procyclic form of T. brucei.
|Category: Journal Article|
|PubMed ID: #17567955|
|Includes FDA Authors from Scientific Area(s): Biologics|
|Entry Created: 2011-10-04||Entry Last Modified: 2012-08-29|