Scientific Publications by FDA Staff

Nat Struct Mol Biol 2008 Aug;15(8):868-9

NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B.

Zhou Z, Feng H, Hansen DF, Kato H, Luk E, Freedberg DI, Kay LE, Wu C, Bai Y

Bai, YW, NCI, Biochem & Mol Biol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA NCI, Biochem & Mol Biol Lab, Ctr Canc Res, NIH, Bethesda, MD 20892 USA Univ Toronto, Dept Med Genet Biochem & Chem, Toronto, ON M5S 1A8, Canada US FDA, NMR Spect Lab Bacterial Polysaccharides, Ctr Biol Evaluat & Res, Bethesda, MD 20892 USA

The NMR structure of budding yeast chaperone Chz1 complexed with histones H2A.Z-H2B has been determined. Chz1 forms a long irregular chain capped by two short alpha-helices, and uses both positively and negatively charged residues to stabilize the histone dimer. A molecular model that docks Chz1 onto the nucleosome has implications for its potential functions.