Scientific Publications by FDA Staff

Clin Vaccine Immunol 2009 Jan;16(1):122-6

Early pulmonary cytokine and chemokine responses in mice immunized with three different vaccines against Mycobacterium tuberculosis determined by PCR array.

Lim J, Derrick SC, Kolibab K, Yang AL, Porcelli S, Jacobs WR, Morris SL

In this study, the early pulmonary cytokine and chemokine responses in mice immunized with either BCG vaccine, a DeltasecA2 mutant of M. tuberculosis, or a DNA vaccine expressing an ESAT6/Antigen 85B fusion protein and then aerogenically challenged with a low dose of M. tuberculosis were evaluated by PCR array. The cellular immune responses at day 10 post-challenge were essentially equivalent in the lungs of mice immunized with either the highly immunogenic BCG vaccine or the DeltasecA2 M. tuberculosis mutant strain. Specifically, 12 immune bio-molecules (including IFN-gamma, IL21, IL27, IL17f, CXCL9, CXCL10, and CXCL11) were differentially regulated, relative to naïve controls, in the lungs of vaccinated mice at this time point. Although the vaccine-related immune responses evoked in mice immunized with the DNA vaccine were relatively limited at 10 days post-infection, the up-regulation of IFN-gamma RNA synthesis as well as the increased expression of CXCL9, CXCL10, and CXCL11 chemokines were detected.