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U.S. Department of Health and Human Services

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J Virol 2009 May;83(9):4023-9

Optimizing viral protein yield of influenza virus strain A/Vietnam/1203/2004 by modification of the neuraminidase gene.

Adamo JE, Liu T, Schmeisser F, Ye Z


Preparation of high yield pre-pandemic influenza H5N1 strains has presented a challenge to both researchers and vaccine manufacturers. The reasons for relatively low yield of the H5N1 strains are not fully understood, but might be partially dependent on the interactions between the hemagglutinin (HA) or neuraminidase (NA) surface glycoproteins with other influenza viral proteins. In this study we have constructed chimeras between the A/Puerto Rico/8/34 (PR8) NA gene and the A/Vietnam/1203/2004 (VN1203) NA gene which have resulted in an increase in virus yield of the reassortant viruses without significant loss of NA activity. By combining the amino-terminus of NA from the PR8 strain, with the carboxy-terminus of NA from VN1203, the surface epitopes unique to the H5N1 VN1203 NA glycoprotein are maintained. This reassortant virus, had a higher titer and total protein yield in eggs, grew to a higher titer, produced large plaques on MDCK cells, and retained NA activity. This work describes a novel recombinant technique designed to increase yields of vaccine candidates for production of pandemic influenza vaccines. The relationship between the infectivity and protein yield of the reassortants is also discussed.

Category: Journal Article
PubMed ID: #19224999 DOI: 10.1128/JVI.02391-08
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2011-10-04 Entry Last Modified: 2012-08-29