Scientific Publications by FDA Staff
Curr Protoc Immunol 2011 Apr;Chapter 14:Unit14.25
Measurement of macrophage-mediated killing of intracellular bacteria, including Francisella and mycobacteria.
Elkins KL, Cowley SC, Conlan JW
Macrophages activated by T cell cytokines are a critical defense mechanism against intracellular bacterial pathogens. This unit presents two general methods for assessing the capacity of mouse macrophages, activated with either soluble cytokines or whole immune T lymphocytes, to control or reduce numbers of intracellular bacteria residing within them. "Measurement of killing" is inferred from a reduction in the number of colony-forming units (cfu) of bacteria at the end of a culture period, compared to the input numbers of cfu at initiation of culture, to the peak numbers of cfu measured during culture, or to a control group in which killing is expected to be poor.
|Category: Journal Article|
|PubMed ID: #21462167||DOI: 10.1002/0471142735.im1425s93|
|Includes FDA Authors from Scientific Area(s): Biologics|
|Entry Created: 2011-10-03||Entry Last Modified: 2012-08-29|