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J Virol 2010 Aug;84(16):8342-7

Determinants in 3Dpol modulate the rate of growth of hepatitis A virus.

Konduru K, Kaplan GG

Abstract

Hepatitis A Virus (HAV), an atypical Picornaviridae, grows poorly in cell culture. To define determinants of HAV growth, we introduced a blasticidin resistance gene into the virus genome and selected variants that grew at high concentrations of blasticidin (Bsd). The mutants grew fast, had an increased rate of RNA replication and translation but did not produce significantly higher virus yields. Nucleotide sequence analysis and reverse genetic studies revealed that a T6069G change resulting in a F42L amino acid substitution in the viral polymerase (3D(pol)) was required for growth at high Bsd concentrations whereas a silent C7027T mutation enhanced the growth rate. Here, we identified novel determinant(s) in 3D(pol) that control the kinetic of HAV growth.


Category: Journal Article
PubMed ID: #20534860 DOI: 10.1128/JVI.01470-09
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2011-10-04 Entry Last Modified: 2012-08-29
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