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J Virol 2012 Feb;86(3):1768-76

The V protein of mumps virus plays a critical role in pathogenesis.

Xu P, Luthra P, Li Z, Fuentes S, D'Andrea JA, Wu J, Rubin S, Rota PA, He B

Abstract

Mumps virus (MuV) causes an acute infection in humans characterized by a wide array of symptoms ranging from relatively mild manifestations, such as parotitis, to more-severe complications, such as meningitis and encephalitis. Widespread mumps vaccination has reduced mumps incidence dramatically; however, outbreaks still occur in vaccinated populations. The V protein of MuV, when expressed in cell culture, blocks interferon (IFN) expression and signaling and interleukin-6 (IL-6) signaling. In this work, we generated a recombinant MuV incapable of expressing the V protein (rMuVDeltaV). The rescued MuV was derived from a clinical wild-type isolate from a recent outbreak in the United States (MuV(Iowa/US/06), G genotype). Analysis of the virus confirmed the roles of V protein in blocking IFN expression and signaling and IL-6 signaling. We also found that the rMuV(Iowa/US/06)DeltaV virus induced high levels of IL-6 expression in vitro, suggesting that V plays a role in reducing IL-6 expression. In vivo, the rMuV(Iowa/US/06)DeltaV virus was highly attenuated, indicating that the V protein plays an essential role in viral virulence.


Category: Journal Article
PubMed ID: #22090137 DOI: 10.1128/JVI.06019-11
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2012-02-20 Entry Last Modified: 2012-08-29
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