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J Clin Microbiol 2012 Aug;50(8):2770-3

Identification of DNA signatures suitable for use in development of real-time PCR assays by whole-genome sequence approaches: use of Streptococcus pyogenes in a pilot study.

Hung GC, Nagamine K, Li B, Lo SC

Abstract

A stepwise computational approach using three layers of publicly available software was described to effectively identify DNA signatures for Streptococcus pyogenes. PCR testing validated 9 out of 15 signature-derived primer sets could detect as low as 5 fg of target DNA with high specificity. The selected signature-derived primer sets were successfully evaluated against all 23 clinical isolates. The approach is readily applicable for designing molecular assays for rapid detection and characterization of various pathogenic bacteria.


Category: Journal Article
PubMed ID: #22593599 DOI: 10.1128/JCM.01155-12
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2012-03-24 Entry Last Modified: 2012-12-10
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