Scientific Publications by FDA Staff
PLoS Pathog 2012 Feb;8(2):e1002536
Efficient capture of infected neutrophils by dendritic cells in the skin inhibits the early anti-leishmania response.
Ribeiro-Gomes FL, Peters NC, Debrabant A, Sacks DL
Neutrophils and dendritic cells (DCs) converge at localized sites of acute inflammation in the skin following pathogen deposition by the bites of arthropod vectors or by needle injection. Prior studies in mice have shown that neutrophils are the predominant recruited and infected cells during the earliest stage of Leishmania major infection in the skin, and that neutrophil depletion promotes host resistance to sand fly transmitted infection. How the massive influx of neutrophils aimed at wound repair and sterilization might modulate the function of DCs in the skin has not been previously addressed. The infected neutrophils recovered from the skin expressed elevated apoptotic markers compared to uninfected neutrophils, and were preferentially captured by dermal DCs when injected back into the mouse ear dermis. Following challenge with L. major directly, the majority of the infected DCs recovered from the skin at 24 hr stained positive for neutrophil markers, indicating that they acquired their parasites via uptake of infected neutrophils. When infected, dermal DCs were recovered from neutrophil depleted mice, their expression of activation markers was markedly enhanced, as was their capacity to present Leishmania antigens ex vivo. Neutrophil depletion also enhanced the priming of L. major specific CD4(+) T cells in vivo. The findings suggest that following their rapid uptake by neutrophils in the skin, L. major exploits the immunosuppressive effects associated with the apoptotic cell clearance function of DCs to inhibit the development of acquired resistance until the acute neutrophilic response is resolved.
|Category: Journal Article|
|PubMed ID: #22359507||DOI: 10.1371/journal.ppat.1002536|
|PubMed Central ID: #PMC3280984|
|Includes FDA Authors from Scientific Area(s): Biologics|
|Entry Created: 2012-03-26||Entry Last Modified: 2012-08-29|