Scientific Publications by FDA Staff
PLoS One 2013;8(2):e56527
Interferon-alpha Is the Primary Plasma Type-I IFN in HIV-1 Infection and Correlates with Immune Activation and Disease Markers.
Hardy GA, Sieg S, Rodriguez B, Anthony D, Asaad R, Jiang W, Mudd J, Schacker T, Funderburg NT, Pilch-Cooper HA, Debernardo R, Rabin RL, Lederman MM, Harding CV
Type-I interferon (IFN-I) has been increasingly implicated in HIV-1 pathogenesis. Various studies have shown elevated IFN-I and an IFN-I-induced gene and protein expression signature in HIV-1 infection, yet the elevated IFN-I species has not been conclusively identified, its source remains obscure and its role in driving HIV-1 pathogenesis is controversial. We assessed IFN-I species in plasma by ELISAs and bioassay, and we investigated potential sources of IFN-I in blood and lymph node tissue by qRT-PCR. Furthermore, we measured the effect of therapeutic administration of IFN¿ in HCV-infected subjects to model the effect of IFN¿ on chronic immune activation. IFN-I bioactivity was significantly increased in plasma of untreated HIV-1-infected subjects relative to uninfected subjects (p¿=¿0.012), and IFN¿ was the predominant IFN-I subtype correlating with IFN-I bioactivity (r¿=¿0.658, p<0.001). IFN¿ was not detectable in plasma of subjects receiving anti-retroviral therapy. Elevated expression of IFN¿ mRNA was limited to lymph node tissue cells, suggesting that peripheral blood leukocytes are not a major source of IFN¿ in untreated chronic HIV-1 infection. Plasma IFN-I levels correlated inversely with CD4 T cell count (p¿=¿0.003) and positively with levels of plasma HIV-1 RNA and CD38 expression on CD8 T cells (p¿=¿0.009). In hepatitis C virus-infected subjects, treatment with IFN-I and ribavirin increased expression of CD38 on CD8 T cells (p¿=¿0.003). These studies identify IFN¿ derived from lymph nodes, rather than blood leukocytes, as a possible source of the IFN-I signature that contributes to immune activation in HIV-1 infection.
|Category: Journal Article|
|PubMed ID: #23437155||DOI: 10.1371/journal.pone.0056527|
|PubMed Central ID: #PMC3577907|
|Includes FDA Authors from Scientific Area(s): Biologics|
|Entry Created: 2012-03-28||Entry Last Modified: 2013-04-06|