• Decrease font size
  • Return font size to normal
  • Increase font size
U.S. Department of Health and Human Services

Scientific Publications by FDA Staff

  • Print
  • Share
  • E-mail

Search Publications



Starting Date

Ending Date

Order by

Entry Details

J Virol Methods 2012 Aug;183(2):219-23

Application of a full-genome microarray-based assay for the study of genetic variability of West Nile virus.

Grinev A, Lu Z, Chizhikov V, Rios M


Viral adaptation through fixation of spontaneous mutations is an important factor potentially associated with reoccurrence of West Nile virus (WNV) outbreaks in the New World. The emergence of new genetic variants of WNV represents an important public health issue because it may affect the sensitivity of WNV screening and diagnostic assays, as well as the development and efficacy of WNV vaccines and anti-viral drugs. A microarray assay was developed and optimized to enable simple monitoring of WNV genetic variability and rapid detection of any nucleotide mutations within the entire viral genome. The assay was validated using 11 WNV isolates from the 2007 and 2009 U.S. epidemics. The developed microarray system can potentially serve as a high throughput, rapid, and effective approach for the detection of circulating WNV genetic variants.

Category: Journal Article
PubMed ID: #22561983 DOI: 10.1016/j.jviromet.2012.04.001
Includes FDA Authors from Scientific Area(s): Biologics
Entry Created: 2012-05-09 Entry Last Modified: 2012-10-02