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J Microcolumn Sep 1995 Sep-Oct;7(5):451-4

Analysis of fumonisin B1 in corn by capillary electrophoresis with fluorescence detection of the FMOC derivative

Holcomb M, Thompson HC Jr

Abstract

A new method has been developed for the analysis of fumonisin B1 in corn using capillary electrophoresis. Fumonisin B1 is the major fumonisin metabolite produced by the fungus Fusarium moniliforme and has been implicated in human and animal diseases. Fumonisin B1 was extracted from corn with acetonitrile/water (50/50) and cleaned up with a Waters C18 Sep-Pak Vac cartridge. Fumonisin B1 was quantitated after elution from the C18 Sep-Pak column using capillary electrophoresis (CE) with a 25 mM sodium borate buffer (pH 9.0) containing 10% acetonitrile and fluorescence detection of the FMOC derivative. The minimum detectable amount in corn was 0.5 ppm. Recovery values in spiked corn averaged over 96% over the range 1¿20 ppm.


Category: Journal Article
DOI: 10.1002/mcs.1220070503
Includes FDA Authors from Scientific Area(s): Toxicological Research
Entry Created: 2012-11-12
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