Scientific Publications by FDA Staff
Photochem Photobiol 1994 Sep;60(3):225-30
Photoactivated gilvocarcin V induces DNA-protein crosslinking in genes for human ribosomal RNA and dihydrofolate reductase.
Matsumoto A, Fujiwara Y, Elespuru RK, Hanawalt PC
The nature of DNA interactions with photoactivated gilvocarcin V has been analyzed at the gene level in both rRNA and dihydrofolate reductase genes of human fibroblasts, utilizing a modified Southern hybridization technique. Neither interstrand DNA crosslinking nor RNA linkage to DNA was detected. However, we consistently observed in both genes retarded DNA bands appearing in a dose-dependent fashion following exposure to photoactivated gilvocarcin V. These retarded bands were enhanced when genomic DNA was prepared without proteinase K treatment, suggesting involvement of protein in this DNA interaction. Because these bands disappear following proteinase K treatment, it is probable that photoactivated gilvocarcin V induces DNA-protein crosslinking.
|Category: Journal Article|
|PubMed ID: #7972373||DOI: 10.1111/j.1751-1097.1994.tb05095.x|
|Includes FDA Authors from Scientific Area(s): Medical Devices|
|Entry Created: 2012-11-13|