Scientific Publications by FDA Staff

Appl Environ Microbiol 2013 Jan;79(2):734-7

A multiplex PCR assay, targeting a di-guanylate cyclase encoding gene, cdgc1 to differentiate species within the genus Cronobacter.

Carter L, Lindsey LA, Grim CJ, Sathyamoorthy V, Jarvis KG, Gopinath G, Lee C, Sadowski JA, Trach L, Pava-Ripoll M, McCardell BA, Tall BD, Hu L

Compared to the widely used Cronobacter rpoB-PCR assay, a highly specific multiplexed PCR assay, based on cdgc1, a di-guanylate cyclase gene was designed that identified all of the targeted six species among 305 Cronobacter isolates. This assay will be a valuable tool for identifying suspected Cronobacter isolates from foodborne investigations.