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J Rapid Meth Aut Mic 1997 May;5(2):139-49

A Modified Anaerogen System for Growth of Campylobacter Spp

Tran TT, Yin JJ

Abstract

A simple, rapid procedure to generate an oxygen-reduced atmosphere suitable for growing Campylobacter jejuni was investigated. A modified AnaeroGenTM system (MAS), consisting of a single AnaeroGenTM anaerobic sachet AM35 (Oxoid Unipath Ltd., Basingstoke, UK) activated in a 9 L anaerobic jar (BBL, Cockeysville, MD), was evaluated and compared with the conventional gassed jar system described in the 8th edition of the U.S. Food and Drug Administration's Bacteriological Analytical Manual (BAM). Enriched cultures of C. jejuni (six replicates at each of five inoculum levels: 10-2 to 102 cfu/mL in artificially contaminated raw milk, raw oyster, crab meat, or mushroom were streaked in duplicate onto four different selective isolation agars for simultaneous incubation in MAS and in the BAM system. No significant differences (p > 0. 05) in recovery rates of C. jejuni were observed for the two systems. The type of isolation agar used did not affect these recovery rates. The quality of growth of C. jejuni at 24, 48 or 72 h was similar in both systems. MAS successfully reduced atmospheric oxygen to a level suitable for the growth of C. jejuni. It was simple and rapid compared to the BAM system, and cost-effective compared to the Oxoid CampyGenTM system specifically designed for the growth of Campylobacter spp.


Category: Journal Article
DOI: 10.1111/j.1745-4581.1997.tb00156.x
Includes FDA Authors from Scientific Area(s): Food
Entry Created: 2012-12-26
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