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Biotechnol Prog 2013 May;29(3):745-53

Fermentanomics informed amino acid supplementation of an antibody producing mammalian cell culture.

Read EK, Bradley SA, Smitka TA, Agarabi CD, Lute SC, Brorson KA

Abstract

Fermentanomics, or a global understanding of a culture state on the molecular level empowered by advanced techniques like NMR, was employed to show that a model hybridoma culture supplied with glutamine and glucose depletes aspartate, cysteine, methionine, tryptophan, and tyrosine during antibody production. Supplementation with these amino acids prevents depletion and improves culture performance. Furthermore, no significant changes were observed in the distribution of glycans attached to the IgG3 in cultures supplemented with specific amino acids, arguing that this strategy can be implemented without fear of impacts on important product quality attributes. In summary, a targeted strategy of quantifying media components and designing a supplementation strategy can improve bioprocess cell cultures when enpowered by fermentanomics tools. (c) 2013 American Institute of Chemical Engineers Biotechnol. Prog., 2013.


Category: Journal Article
PubMed ID: #23606649
Includes FDA Authors from Scientific Area(s): Drugs
Entry Created: 2013-03-20 Entry Last Modified: 2014-01-07
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