Scientific Publications by FDA Staff

Microbes Infect 2013 Nov;15(12):816-27

Generation of protection against Francisella novicida in mice depends on the pathogenicity protein PdpA, but not PdpC or PdpD.

Chou AY, Gordon NK, Nix EB, Schmerk CL, Nano FE, Elkins KL

Previous results suggest that mutations in most genes in the Francisella pathogenicity island (FPI) attenuate the bacterium. Using a mouse model, here we determined the impact of mutations in pdpA, pdpC, and pdpD in Francisella novicida on in vitro replication in macrophages, and in vivo immunogenicity. In contrast to most FPI genes, deletion of pdpC (Fn¿pdpC) and pdpD (Fn¿pdpD) from F. novicida did not impact growth in mouse bone-marrow derived macrophages. Nonetheless, both Fn¿pdpC and Fn¿pdpD were highly attenuated when administered intradermally. Infected mice produced relatively normal anti-F. novicida serum antibodies. Further, splenocytes from infected mice controlled intramacrophage Francisella replication, indicating T cell priming, and mice immunized by infection with Fn¿pdpC or Fn¿pdpD survived secondary lethal parenteral challenge with either F. novicida or F. tularensis LVS. In contrast, deletion of pdpA (Fn¿pdpA) ablated growth in macrophages in vitro. Fn¿pdpA disseminated and replicated poorly in infected mice, accompanied by development of some anti-F. novicida serum antibodies. However, primed Th1 cells were not detected, and vaccinated mice did not survive even low dose challenge with either F. novicida or LVS. Taken together, these results suggest that successful priming of Th1 cells, and protection against lethal challenge, depends on expression of PdpA.