Scientific Publications by FDA Staff
Anal Meth 2013 Jun 7;5(11):2657-9
Separation and identification of phosphorothioate oligonucleotides by HILIC-ESIMS
Easter R, Barry C, Caruso J, Limbach P
A combined hydrophilic interaction chromatography (HILIC) electrospray ionization mass spectrometry (ESI-MS) approach for the separation and identification of phosphorothioate oligonucleotides is described. Phosphorothioate 21-mer and 23-mer were separated by HILIC and detected using selected ion monitoring (SIM) ESI-MS. Phosphorothioates could be detected from 50 nM solutions suggesting effectiveness comparable to ion pairing reversed phase chromatography approaches.
|Category: Journal Article|
|Includes FDA Authors from Scientific Area(s): Regulatory Affairs|
|Entry Created: 2013-06-23|