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U.S. Department of Health and Human Services

Scientific Publications by FDA Staff

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Anal Meth 2013 Jun 7;5(11):2657-9

Separation and identification of phosphorothioate oligonucleotides by HILIC-ESIMS

Easter R, Barry C, Caruso J, Limbach P


A combined hydrophilic interaction chromatography (HILIC) electrospray ionization mass spectrometry (ESI-MS) approach for the separation and identification of phosphorothioate oligonucleotides is described. Phosphorothioate 21-mer and 23-mer were separated by HILIC and detected using selected ion monitoring (SIM) ESI-MS. Phosphorothioates could be detected from 50 nM solutions suggesting effectiveness comparable to ion pairing reversed phase chromatography approaches.

Category: Journal Article
DOI: 10.1039/c3ay26519f
Includes FDA Authors from Scientific Area(s): Regulatory Affairs
Entry Created: 2013-06-23