Scientific Publications by FDA Staff
Microb Drug Resist 2013 Jun 21 [Epub ahead of print]
Effect of Fluoroquinolone Resistance Selection on the Fitness of Three Strains of Clostridium perfringens.
Park M, Sutherland JB, Kim JN, Rafii F
Selection of bacterial strains for resistance to antimicrobial agents may affect fitness; and fluoroquinolone resistance has been shown to affect fitness of aerobic and facultative bacteria. The impact on bacterial fitness of resistance selection to three fluoroquinolones was examined in three wild-type strains of Clostridium perfringens: ATCC 13124, ATCC 3626, and NCTR. Selection for resistance to norfloxacin, ciprofloxacin, and gatifloxacin affected the fitness of nine mutant strains differently. In a series of pure cultures grown in the absence of drugs, the growth of each of the mutants was comparable to that of the corresponding wild type. In competition experiments between mutants and isogenic wild types, however, some of the mutants were less fit. The fitness of ciprofloxacin-resistant mutants was comparable for all three strains, but two gatifloxacin-resistant mutants and one norfloxacin-resistant mutant were significantly less fit than the corresponding wild types. We conclude that both the genetic background of the strain and the fluoroquinolone which induced resistance affected the fitness of the resistant mutants. This is the first time that the effect on fitness of resistance to antimicrobial agents has been measured for C. perfringens.
|Category: Journal Article|
|PubMed ID: #23789809||DOI: 10.1089/mdr.2013.0056|
|Includes FDA Authors from Scientific Area(s): Toxicological Research|
|Entry Created: 2013-06-25|