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PLoS One 2014 Apr 9;9(4):e94254

An integrated flow cytometry-based system for real-time, high sensitivity bacterial detection and identification.

Buzatu DA, Moskal TJ, Williams AJ, Cooper WM, Mattes WB, Wilkes JG


Foodborne illnesses occur in both industrialized and developing countries, and may be increasing due to rapidly evolving food production practices. Yet some primary tools used to assess food safety are decades, if not centuries, old. To improve the time to result for food safety assessment a sensitive flow cytometer based system to detect microbial contamination was developed. By eliminating background fluorescence and improving signal to noise the assays accurately measure bacterial load or specifically identify pathogens. These assays provide results in minutes or, if sensitivity to one cell in a complex matrix is required, after several hours enrichment. Conventional assessments of food safety require 48 to 56 hours. The assays described within are linear over 5 orders of magnitude with results identical to culture plates, and report live and dead microorganisms. This system offers a powerful approach to real-time assessment of food safety, useful for industry self-monitoring and regulatory inspection.

Category: Journal Article
PubMed ID: #24718659 DOI: 10.1371/journal.pone.0094254
PubMed Central ID: #PMC3981798
Includes FDA Authors from Scientific Area(s): Toxicological Research
Entry Created: 2014-04-12 Entry Last Modified: 2014-05-24