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J AOAC Int 2014 May-Jun;97(3):889-95
Screening Multimycotoxins in Food-Grade Gums by Stable Isotope Dilution and Liquid Chromatography/Tandem Mass Spectrometry.
Zhang K, Wong JW, Jia ZW, Vaclavikova M, Trucksess MW, Begley TH
Abstract
Stable isotope dilution with LC/MS/MS was used to determine the following 11 mycotoxins in food grade gums: aflatoxins B-1, B-2, G(1), and G(2); deoxynivalenol; fumonisins B-1, B-2, and B-3; ochratoxin A; T-2 toxin; and zearalenone. Samples were fortified with 11 [C-13]-uniformly labeled internal standard ([C-13]-IS) mycotoxins that corresponded to the 11 target mycotoxins and extracted by acetonitrile-water (4 + 1, v/v), followed by LC/MS/MS analysis. Mycotoxins were quantitated with the fortified (C-13]-IS in each sample. The average recoveries of aflatoxins B-1, B-2, G(1), and G(2) (1, 5, and 25 mu g/kg); deoxynivalenol and fumonisins B-1, B-2, and B-3 (25, 100, and 500 mu g/kg); and ochratoxin A, T-2 toxin, and zearalenone (10, 50, and 250 mu g/kg) ranged from 84 to 117% with RSDs less than 20%. Method-dependent LOQs were from 0.1 (aflatoxin B-1) to 25 mu g/kg (fumonisin B-3). Among 20 market samples, aflatoxin B-1 (
Category:
Journal Article
DOI:
10.5740/jaoacint.13-263
Includes FDA Authors from Scientific Area(s):
Food
Entry Created:
2014-07-19
Entry Last Modified:
2018-09-23