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J Microbiol Methods 2015 Feb 14 [Epub ahead of print]

Identification of five Shiga toxin-producing Escherichia coli genes by Luminex microbead-based suspension array.

Son I, Binet R, Lin A, Hammack TS, Kase JA

Abstract

To rapidly identify the presence of potentially virulent O157:H7 and non-O157 Shiga toxin-producing Escherichia coli (STEC), a PCR-based Luminex suspension assay was developed to detect the genes coding for four virulence factors (stx1, stx2, eae, and ehxA) plus the O157:H7-specific +93 uidA single nucleotide polymorphism.

Category: Journal Article
PubMed ID: #25686714 DOI: 10.1016/j.mimet.2015.02.008
Includes FDA Authors from Scientific Area(s): Food Regulatory Affairs
Entry Created: 2015-02-18
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