Scientific Publications by FDA Staff

Int J Infect Dis 2015 Aug;37:152-8

Genetic relatedness of selected clinical and environmental non-O1/O139 Vibrio cholerae.

Aydanian A, Tang L, Chen Y, Morris JJ, Olsen P, Johnson JA, Nair GB, Stine OC

BACKGROUND: In an attempt to better understand the non-O1/O139 isolates of V. cholerae, we undertook a systematic study of clinical and environmental isolates collected from various geographical locations collected between the years 1932 and 1998. METHODS: Ninety-nine Vibrio cholerae isolates collected from clinical and environmental sources from various geographical regions between 1932 and 1998 were studied by sequencing seven housekeeping genes. Genetic relatedness was defined by multiple methods that allow for the observed high levels of recombination. RESULTS: We determined four V. cholerae subpopulations. One subpopulation contained mostly environmental isolates; a second, the cholera-toxin-positive serogroup O1/O139 isolates; and the other two subpopulations were enriched for non-O1/O139 clinical isolates that were frequently clonally related to each other. CONCLUSIONS: Our data suggest that many of these non-O1/O139 clinical isolates were phylogenetically related to common ancestors, even though the isolates had been collected from up to 36 years apart and from different countries or continents.